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在单因素实验的基础上，采用响应面法优化了蒲桃种子总黄酮的超声辅助乙醇提取工艺，采用大孔树脂吸附法对总黄酮进行纯化制备了蒲桃种子精制总黄酮，并评价了纯化前后总黄酮的体外抗氧化活性和降血糖活性。结果表明，蒲桃种子总黄酮的最佳提取工艺为：液料比42∶1(mL∶g)、乙醇体积分数57%、超声时间30 min、超声温度62℃,总黄酮得率为(5.500±0.026)%,与预测值(5.51%)接近，纯度为(16.02±0.56)%;最佳纯化工艺为：选择大孔树脂S-8、上样液质量浓度2.0 mg·mL-1、上样液pH值3、上样体积50 mL、洗脱剂乙醇体积分数70%、洗脱体积100 mL,在此条件下，精制总黄酮纯度提高至(34.97±0.18)%,较纯化前提高了1.2倍；精制总黄酮的DPPH自由基清除能力[IC50=(9.36±0.95)μg·mL-1]、铁离子还原能力及对α-葡萄糖苷酶[IC50=(135.01±7.07)μg·mL-1]、α-淀粉酶[IC50=(28.45±0.89)μg·mL-1)的抑制能力均强于总黄酮粗提物，精制总黄酮的ABTS自由基清除能力弱于总黄酮粗提物，精制总黄酮对α-葡萄糖苷酶、α-淀粉酶的抑制类型均为非竞争性。为蒲桃种子总黄酮开发为抗氧化降血糖功能性产品提供了科学依据。
Abstract：On the basis of single-factor experiments, we optimized the ultrasound-assisted ethanol extraction process of total flavonoids from Syzygium jambos L.Alston seeds by response surface methodologies, and purified total flavonoids by macroporous resin adsorption to obtain the refined total flavonoids.Moreover, we evaluated the in vitro antioxidant activity and hypoglycemic activity of total flavonoids before and after purification.The results show that the optimal extraction process is obtained as follows: the liquid-solid ratio of 42∶1(mL∶g),the ethanol volume fraction of 57%,the ultrasonic time of 30 min, and the ultrasonic temperature of 62 ℃,the yield of total flavonoids is(5.500±0.026)%,which is close to the predicted value(5.51%),and the purity is(16.02±0.56)%.The optimal purification process is obtained as follows: using macroporous resin S-8,the mass concentration of the sample solution of 2.0 mg·mL-1,the sample solution pH value of 3,the sample volume of 50 mL,the ethanol volume fraction of 70% as eluent, and the elution volume of 100 mL.Under above conditions, the purity of refined total flavonoids increases to(34.97±0.18)%,which is 1.2 times higher than that before purification.The DPPH free radicals scavenging ability[IC50=(9.36±0.95) μg·mL-1],iron ion reducing ability, α-glucosidase inhibitory ability[IC50=(135.01±7.07) μg·mL-1],and α-amylase inhibitory ability[IC50=(28.45±0.89) μg·mL-1] of the refined total flavonoids are stronger than those of the crude extract of total flavonoids.The ABTS free radicals scavenging ability of the refined total flavonoids is weaker than that of the crude extract of total flavonoids.The refined total flavonoids shows non-competitive inhibition on α-glucosidase and α-amylase.This study provides a scientific basis for the development of total flavonoids from Syzygium jambos L.Alston seeds as antioxidant and hypoglycemic functional products.
[1] MAGLIANO D J,BOYKO E J.IDF Diabetes Atlas[M].IDF Diabetes Atlas 10th edition scientific committee,2021.
[2] GLOVACI D,FAN W J,WONG N D,et al.Epidemiology of diabetes mellitus and cardiovascular disease[J].Current Cardiology Reports,2019,21(4):21.
[3] ETASASSALA N G E R,BADMUS J A,MARNEWICK J L,et al.Alpha-glucosidase and alpha-amylase inhibitory activities,molecular docking,and antioxidant capacities of Plectranthus ecklonii constituents[J].Antioxidants,2022,11(2):378.
[4] PROENÇA C,RIBEIRO D,FREITAS M,et al.Flavonoids as potential agents in the management of type 2 diabetes through the modulation of α-amylase and α-glucosidase activity:a review[J].Critical Reviews in Food Science and Nutrition,2022,62(12):3137-3207.
[5] GONG L X,FENG D N,WANG T X,et al.Inhibitors of α-amylase and α-glucosidase:potential linkage for whole cereal foods on prevention of hyperglycemia[J].Food Science & Nutrition,2020,8(12):6320-6337.
[6] NEWMAN D J,CRAGG G M.Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019[J].Journal of Natural Products,2020,83(3):770-803.
[7] 林大都，成金乐，彭丽华，等.蒲桃的研究进展[J].安徽农业科学，2015,43(10):76-78.LIN D D,CHENG J L,PENG L H,et al.Research progress of Syzygium jambos[J].Journal of Anhui Agricultural Sciences,2015,43(10):76-78.
[8] OCHIENG M A,BEN W B,BITCHAGNO G T M,et al.Syzygium jambos L.Alston:an insight into its phytochemistry,traditional uses,and pharmacological properties[J].Frontiers in Pharmacology,2022,13:786712.
[9] ZULCAFLI A S,LIM C,LING A P,et al.Antidiabetic potential of Syzygium sp.:an overview[J].Yale Journal of Biology and Medicine,2020,93(2):307-325.
[10] HOSSAIN H,RAHMAN S E,AKBAR P N,et al.HPLC profiling,antioxidant and in vivo anti-inflammatory activity of the ethanol extract of Syzygium jambos available in Bangladesh[J].BMC Research Notes,2016,9:191.
[11] ISLAM M R,PARVIN M S,ISLAM M E.Antioxidant and hepatoprotective activity of an ethanol extract of Syzygium jambos(L.) leaves[J].Drug Discoveries & Therapeutics,2012,6(4):205-211.
[12] JAYASINGHE U L B,RATNAYAKE R M S,MEDAWALA M M W S,et al.Dihydrochalcones with radical scavenging properties from the leaves of Syzygium jambos[J].Natural Product Research,2007,21(6):551-554.
[13] AMIR RAWA M S,NURUL AZMAN N A,MOHAMAD S,et al.In vitro and in silico anti-acetylcholinesterase activity from Macaranga tanarius and Syzygium jambos[J].Molecules,2022,27(9):2648.
[14] SOBEH M,ESMAT A,PETRUK G,et al.Phenolic compounds from Syzygium jambos(Myrtaceae) exhibit distinct antioxidant and hepatoprotective activities in vivo[J].Journal of Functional Foods,2018,41:223-231.
[15] ROCCHETTI G,LUCINI L,AHMED S R,et al.In vitro cytotoxic activity of six Syzygium leaf extracts as related to their phenolic profiles:an untargeted UHPLC-QTOF-MS approach[J].Food Research International,2019,126:108715.
[16] QIAO Z,HAN L,LIU X S,et al.Extraction,radical scavenging activities,and chemical composition identification of flavonoids from sunflower(Helianthus annuus L.) receptacles[J].Molecules,2021,26(2):403.
[17] ZHAO K,CHEN M,LIU T,et al.Rhizoma drynariae total flavonoids inhibit the inflammatory response and matrix degeneration via MAPK pathway in a rat degenerative cervical intervertebral disc model[J].Biomedicine & Pharmacotherapy,2021,138:111466.
[18] MAHMOUD M F,ABDELAAL S,MOHAMMED H O,et al.Syzygium jambos extract mitigates pancreatic oxidative stress,inflammation and apoptosis and modulates hepatic IRS-2/AKT/GLUT4 signaling pathway in Streptozotocin-induced diabetic rats[J].Biomedicine & Pharmacotherapy,2021,142:112085.
[19] WAMBA B E N,NAYIM P,MBAVENG A T,et al.Syzygium jambos displayed antibacterial and antibiotic-modulating activities against resistant phenotypes[J].Evidence-Based Complementary and Alternative Medicine,2018,2018:5124735.
[20] GAVILLAN-SUAREZ J,AGUILAR-PEREZ A,RIVERA-ORTIZ N,et al.Chemical profile and in vivo hypoglycemic effects of Syzygium jambos,Costus speciosus and Tapeinochilos ananassae plant extracts used as diabetes adjuvants in Puerto Rico[J].BMC Complementary Medicine and Therapies,2015,15:244.
[21] 梁子昌，陈明，李大宁，等.响应面法优化牛大力茎总黄酮提取工艺[J].化学与生物工程，2024,41(12):30-35.LIANG Z C,CHEN M,LI D N,et al.Optimization in extraction process of total flavonoids from Millettia speciosa Champ.stem by response surface methodology[J].Chemistry & Bioengineering,2024,41(12):30-35.
[22] ZHANG S T,ZHANG L G,WANG L,et al.Total phenols,flavonoids,and procyanidins levels and total antioxidant activity of different Korean pine(Pinus koraiensis) varieties[J].Journal of Forestry Research,2019,30(5):1743-1754.
[23] 谢海伟，郑柏曦，陈勇智.响应面法优化石榴皮总黄酮提取工艺及其抗氧化性能研究[J].化学与生物工程，2023,40(7):11-17.XIE H W,ZHENG B X,CHEN Y Z.Optimization in extraction process of total flavonoids from pomegranate peel by response surface methodology and its antioxidant property[J].Chemistry & Bioengineering,2023,40(7):11-17.
[24] ZHANG H J,LI H Z,ZHANG Z J,et al.Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology:characterization and in vitro antioxidant activities[J].Journal of Food Science,2021,86(2):306-318.
[25] WANG X Y,SU J Q,CHU X L,et al.Adsorption and desorption characteristics of total flavonoids from Acanthopanax senticosus on macroporous adsorption resins[J].Molecules,2021,26(14):4162.
[26] WANG X Y,WANG S S,HUANG S S,et al.Purification of polyphenols from distiller′s grains by macroporous resin and analysis of the polyphenolic components[J].Molecules,2019,24(7):1284.
[27] WU M,XU J,ZANG H,et al.Purification and identification of flavonoid molecules from Rosa setate x Rosa rugosa waste extracts and evaluation of antioxidant,antiproliferative and antimicrobial activities[J].Molecules,2022,27(14):4379.
[28] 陈庆钥，林阳君，谢永华.辣木叶总黄酮的纯化工艺及抑菌活性研究[J].化学与生物工程，2024,41(8):63-68.CHEN Q Y,LIN Y J,XIE Y H.Purification process of total flavonoids from Moringa oleifera Lam.leaves and its antibacterial activity[J].Chemistry & Bioengineering,2024,41(8):63-68.
[29] 张声源，陈炜铃，王楠，等.六种柚果不同部位矿物质元素、总酚、柚皮苷含量测定及抗氧化活性研究[J].食品与发酵工业，2023,49(18):105-112.ZHANG S Y,CHEN W L,WANG N,et al.Study on mineral elements,total polyphenol,naringin content,and antioxidant activity in different parts of six pomelo cultivars[J].Food and Fermentation Industries,2023,49(18):105-112.
[30] 魏爱红，李晓虹，曾煌，等.枇杷抑制α-葡萄糖苷酶和α-淀粉酶活性部位的筛选及其酶动力学[J].食品与发酵工业，2023,49(5):53-59.WEI A H,LI X H,ZENG H,et al.Screening of effective fraction from Eriobotrya japonica(Thunb.) Lindl with inhibiting activity against α-glucosidase and α-amylase and its inhibition kinetics[J].Food and Fermentation Industries,2023,49(5):53-59.
[31] ZHANG P,SONG Y W,WANG H L,et al.Optimization of flavonoid extraction from Salix babylonica L.buds,and the antioxidant and antibacterial activities of the extract[J].Molecules,2022,27(17):5695.
[32] MYO H,KHAT-UDOMKIRI N.Optimization of ultrasound-assisted extraction of bioactive compounds from coffee pulp using propylene glycol as a solvent and their antioxidant activities[J].Ultrasonics Sonochemistry,2022,89:106127.
[33] XUE H K,TAN J Q,LI Q,et al.Isolation and purification of anthocyanin from blueberry using macroporous resin combined Sephadex LH-20 techniques[J].Food Science and Technology Research,2019,25(1):29-38.
基本信息:
中图分类号:TQ28
引用信息:
[1]庄远杯,聂华,曾煌,等.蒲桃种子总黄酮提取纯化工艺及其抗氧化降血糖活性研究[J].化学与生物工程,2026,43(04):46-56.
基金信息:
国家自然科学基金青年基金项目(81703662); 嘉应学院科研项目(2022KJY15,2021JYUTDY28); 2022年广东省基地嘉应学院客家研究院大客家平台研究团队项目(22KYKT03); 广东省大学生创新创业训练计划项目(202210582418)
2025-08-05
2025-08-05
2025-08-05